MICA Epitope: 14G

Duquesnoy et al, Human Immunology 69: 826-832, 2008 Post-transplant alloserum #2

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT)

Pre-transplant alloserum GP-MIC-1; antibody producer types as MICA*004, *018

Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100. Post-transplant alloserum p5-58 reacted with 14G-carrying MICA*002 and MICA*017; absorption with MICA*002 transfected cells removed all reactivity

Cox, S. T. (2011). The relevance and impact of MICA allele mismatching and MICA antibodies on renal transplantation outcome, University College London (UCL). Ph.D. Dissertation.  The 14G specificity was identified in the sera of 12% of graft recipients positive for MICA antibodies

MICA Epitope: 14W

Duquesnoy, R. J., J. Mostecki, et al. (2008). "A Structurally Based Epitope Analysis of MICA Antibody Specificity Patterns." Human Immunol. 69: 826-832. Two transplant cases with sera with anti-14W associated antibody reactivity

Suarez-Alvarez, B., R. Alonso-Arias, et al. (2009). "Identification of epitopes and immunodominant regions on the MICA protein defined by alloantibodies from kidney transplant patients." Transplantation 88(3 Suppl): S68-77. A MICA antibody positive serum reacted with synthetic MICA peptides which had the 14W residue; this suggested that the 14W-defined epitope might be linear.

Stastny, P. and Y. Zou (2013). "16th IHIW: Report of the MICA project." Int J Immunogenetics 40: 11-16. MICA exchange post-renal transplant sera S009 and S016 (reacted with all nineteen 14W-carrying alleles; there were no false positive or false negative reactions with the 28 MICA allele panel.

MICA Epitope: 24T

Duquesnoy, R. J., J. Mostecki, et al. (2008). "A Structurally Based Epitope Analysis of MICA Antibody Specificity Patterns." Human Immunol. 69: 826-832

Post-transplant alloserum #1 results (equivalent to epitope 25TC)

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT) Alloserum GP-MIC-5

El-Awar, N., P. I. Terasaki, et al. (2007). "Epitopes of the HLA-A, B, C, DR, DQ and MICA antigens." Clinical Transplants: 175-194. Serum eluate URUALB77 (absorbed with MICA*018 cell line) reacted with MICA*001, *012, *018 The epitope was called #6001.

Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100. Post-transplant alloserum Abs224E13 reacted with MICA*001, *012, *018; absorption with MICA*001 transfected cells removed all reactivity.

Cox, S. T. (2011). The relevance and impact of MICA allele mismatching and MICA antibodies on renal transplantation outcome, University College London (UCL). Ph.D. Dissertation. MICA antibody recognition of 24T was observed in 24% of MICA reactive antibodies

MICA Epitope: 36C

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT) Alloserum GP-MIC-6

Post-transplant serum eluate Sample #1 after absorption with MICA*037 positive with MICA*001, *002, *007, *011, *012, *015, *017, *018, *029, *030, *036, *037, *041, *043, *046, *050 (EFI poster, 2009)

Reactivity pattern is similar to previous reports for the “CMGWS supereplet” (Duquesnoy et al, Human Immunology 69: 826-832, 2008) and MICA group G1 (Zou et al, Immunogenetics 61: 91-100, 2009). In each of these earlier reports, limited panels were tested, and positive alleles shared residues 36C, 129M, 206G, 210W and 215S; Zou et al. suggested also that G1 includes 173K). The expanded panel tested by Mostecki showed reactivity directed to 36C; negative reactions were seen with 36Y-carrying MICA*005, *028, *042 and *051, which share one or more of residues129M, 173K, 206G, 210W and 215S.

MICA Epitope: 36Y

Stastny, P. and Y. Zou (2013). "16th IHIW: Report of the MICA project." Int J Immunogenetics 40: 11-16. 16^th Workshop serum S015 reacted with all twelve 36Y-carrying alleles; there were no false positive or false negative reactions with 28 MICA allele panel.

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT) Eluate of serum GP-MIC-4 after absorption with MICA*051:

 Two additional allosera have similar 36Y-specific antibody reactivity patterns (data not shown)

MICA Epitope: 105R

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT) Mouse monoclonal antibody 6D4

MICA Epitope: 122V

Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100. Post-transplant alloserum p10-64 positive with MICA*004, *006, *009; Absorption with MICA*009 transfected cells removed antibody reactivity

El-Awar, N., P. I. Terasaki, et al. (2007). "Epitopes of the HLA-A, B, C, DR, DQ and MICA antigens." Clinical Transplants: 175-194. Called the 122V –defined epitope #6003

Cox, S. T. (2011). The relevance and impact of MICA allele mismatching and MICA antibodies on renal transplantation outcome, University College London (UCL). Ph.D. Dissertation. Transplant case 690MO (Figure 6.5.7) had MICA*004 and MICA*009 antibodies detected and both antigens uniquely express V at residue 122.

MICA Epitope: 125E

Data from Zou et al, Immunogenetics 61: 91-100, 2009: Unnamed post-transplant alloserum tested against a limited panel, positive with MICA*002,  *004, *006, *007, *008, *009, *012, *017, *018, *019

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT) Alloserum GP-MIC-7

MICA Epitope: 125K

Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100. Post-transplant alloserum P5-49 positive with MICA*001, which uniquely carries this epitope

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT)

Alloserum GP-MIC-8

Cox, S. T. (2011). The relevance and impact of MICA allele mismatching and MICA antibodies on renal transplantation outcome, University College London (UCL). Ph.D. Dissertation. Reported antibodies specific for 125K on MICA*001

MICA Epitope: 129M

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT)

Stastny, P. and Y. Zou (2013). "16th IHIW: Report of the MICA project." Int J Immunogenetics 40: 11-16. 16^th Workshop specimen S006 came from a patient following a second renal transplant who typed as MICA*004,*008 ; the second donor typed as MICA*009,*018. The recipient and donor had no mismatches for HLA-A, -B, -DR, but the kidney was rejected one month post-transplant.

MICA Epitope: 151V

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT) Alloserum GP-MIC-12 positive with MICA*011, which uniquely carries this epitope

MICA Epitope: 156H

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT)

MICA Epitope: 156L

Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100. Post-transplant alloserum RJ reacts with MICA*012, which uniquely carries an epitope  reported as 155L

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT)Alloserum GP-MIC-15

MICA Epitope: 173E

Zou, Y., F. Mirbaha, et al. (2002). "MICA is a target for complement-dependent cytotoxicity with mouse monoclonal antibodies and human alloantibodies." Hum Immunol 63: 30-39Alloserum GP-MIC-16  

Serum was not tested for other 173E-carrying alleles (MICA*024, *027).

MICA Epitope: 173K (confirmed) 

Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100. Mapping of 173K-defined epitope detected by antibodies.

Communication from J. Mostecki (Gen-Probe, Stamford, CT:

Stastny, P. and Y. Zou (2013). "16th IHIW: Report of the MICA project." Int J Immunogenetics 40: 11-16. 16^th Workshop specimen S006 came from a patient following a second renal transplant who typed as MICA*004,*008 ; the second donor typed as MICA*009,*018. The recipient and donor had no mismatches for HLA-A, -B, -DR, but the kidney was rejected one month post-transplant. All nineteen 173K-carryting alleles reacted with S006; there were no false positive or false negative reactions with the 28 MICA allele panel

MICA Epitope: 175S

Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100. Post-transplant alloserum p5-31 reactivity.  Mapping of 175S-defined epitope detected by antibodies.

Absorption with MICA*009 removed all reactivity; serum was not tested for other 175S-carrying alleles (MICA*016, *033, *036).

 MICA Epitope: 176I

Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100. Post-transplant alloserum p7-47 reacts only with MICA*006, which uniquely carries this epitope. Mapping of 176I-defined epitope detected by antibodies.

 MICA Epitope: 181R

Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100. Post-transplant alloserum G-495 reacts with MICA*004, which uniquely carries this epitope.   Mapping of 181R-defined epitope detected by antibodies.

El-Awar, N., P. I. Terasaki, et al. (2007). "Epitopes of the HLA-A, B, C, DR, DQ and MICA antigens." Clinical Transplants: 175-194. The 181R-defined epitope was called #6006

Cox, S. T. (2011). The relevance and impact of MICA allele mismatching and MICA antibodies on renal transplantation outcome, University College London (UCL). Ph.D. Dissertation.Transplant case 194AH. The patient was MICA*008,-  and serum reacted  only with MICA*004 in Stastny and Gen-probe kits.  Antibodies may be specific for R at residue 181.

MICA Epitope: 206 GWS

Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100. Mapping of epitope defined by 206G,and 209W with MICA antibodies in sera from transplant patient

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT) GP MFI

11713

50

954

1618

1401

1111

1100

1436

1309

1230

1137

1196

1229

927

1343

1343

1374

1448

90 ± 101

MICA Epitope: 206S₂

 Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100.Post-transplant alloserum 226E03; absorption with MICA*008 and *009 transfected cells removed all reactivity.  Mapping of an epitope defined by 206S and 210R with MICA antibodies in sera from transplant patients

This reactivity pattern was named MICA group 2 (MICA-G2) by Zou et al, defined by residues 36Y, 129V, 173E, 206S, 210R and 215T. To identify the specific epitopes required for a MICA-G2 antibody to react, hybrid recombinants of MICA*002 and MICA*008 were made by replacing residues at positions 173, 206, 210, 215 with residues of the other allele. The hybrid MICA*002 molecule carrying residues 173E/206S/210R/215T reacted with an eluate from MICA-G2-specific serum after absorption with MICA*009 transfected cells, while the hybrid MICA*008 molecule carrying residues of MICA*002 did not, showing that 36Y and 129V were not required for binding. No reactivity was seen with a hybrid recombinant MICA*002 that had 173E substituted for 173K, showing that this position also was not required for binding of that antibody.

MICA Epitope: 213I

Zou, Y., Z. Qin, et al. (2009). "Polymorphisms of MICA recognized by human alloantibodies." Immunogenetics 61(2): 91-100. Antibodies reacted specifically an epitope shared by MICA*008 and MICA*019,  absorption with MICA*008 removed antibody activity. Mapping of a 213I-defined epitope with MICA antibodies in sera from transplant patients

El-Awar, N., P. I. Terasaki, et al. (2007). "Epitopes of the HLA-A, B, C, DR, DQ and MICA antigens." Clinical Transplants: 175-194. This epitope had possible 215I and/or 251R  and was called #6007

Stastny, P. and Y. Zou (2013). "16th IHIW: Report of the MICA project." Int J Immunogenetics 40: 11-16. 16^th IHIW exchange serum S011 came from a transplant recipient who typed as MICA*002, *009; donor typed as MICA*002:01, *08:01 . All six 213I-carrying MICA alleles reacted with S011, there were no false positive or false negative reactions with the 28 MICA allele panel

Data from Justin Mostecki (Immucor, LifeCodes, Stamford, CT) Eluate after absorption with MICA*037 of 16^th IHIW exchange serum S011

Cox, S. T. (2011). The relevance and impact of MICA allele mismatching and MICA antibodies on renal transplantation outcome, University College London (UCL). Ph.D. Dissertation.

Epitope:

213TT (provisional)

Date reported:

26Nov2012/updated 13May2013

Luminex single allele reactivity:

Communication from J. Mostecki (Gen-Probe, Stamford, CT):

Alloserum GP-MIC-21:

Comments:

This epitope is equivalent to eplet 214TT in the HLAMatchmaker program. See also Duquesnoy et al, Human Immunology 69: 826-832, 2008, for a description of this epitope.